Surprising Features of Nuclear Receptor Interaction Networks Revealed by Live Cell Single Molecule Imaging

Raw fast SMT data for Retinoic acid receptor-alpha and Retinoid-X-Receptor-alpha:

Figure1:  Contains fast SMT data for each condition in Figure 1, panel C &D. Two representative SMT movies of each condition is included in the respective subfolders named “Halo_H2B”, “Halo_NLS”, “Rara_c156”, “Rara_c239”, “Rara_c258”, “Rxra_c10”,  “Rxra_D6” and “Rxra_D9”. Subfolder “cdf_plots” contains rbme posterior data used to calculate fraction bound of each Rara and Rxra  endogenous clones. Rara_Rxra_endo_clones_FB.csv shows calculation of chromatin bound fraction from the Rara and Rxra endogenous clones (biological replicates). HaloH2B_HaloNLS_FB.csv shows chromatin bound fraction calculated for Halo-H2B and Halo-NLS using bootstrapping. MarginalPosteriormean_Figure1D.csv corresponds to diffusion spectra in Figure 1D.

Figure2: Two representative SMT movies of Rara and Rxra overexpressed conditions in Figure 2C are included in the respective subfolders named “Rara_O.E” and “Rxra_O.E”. Rara_RXRA_O.E_FB.csv shows chromatin bound fraction calculated for Rara_O.E and Rxra_O.E using bootstrapping. Two representative SMT movies for each condition in Figure 2F are included in their respective subfolders named: “Rara_w/SNAP “, “Rara_w/RARARR-SNAP”, “Rara_w/SNAP-RXRA”, “Rara_w/RARA-SNAP”, “Rxra_w/SNAP”, “Rxra_w/RARARR-SNAP”, “Rxra_w/SNAP-RXRA” and “Rxra_w/RARA-SNAP”. Figure2F_FB.csv shows chromatin bound fraction calculated for all conditions in Figure 2F using bootstrapping.

Figure3: The two subfolders RAR_to_RXR and RXR_to_RAR contain the data from Halo-RAR to SNAPf-RXR and Halo-RXR to SNAPf-RAR PAPA-SMT experiments, respectively. Each folder contains two subfolders: subfolder green_vs_violet contains raw data for Figure 3B plots which show increase in localisations caused by green and violet pules along with the raw data for  residuals in Figure S8B; subfolder “diffusion_spectra” contains marginal posterior vs. diffusion coefficient for both DR and PAPA for the respective conditions shown in Figure 3C and Figure S8A.