Genome wide RNA expression profile of endometrial stromal cells stimulated by cAMP, and cAMP under PGC-1a-knockdown

To know the role of PGC-1a in the change of mRNA expression during desidualization, we compared the mRNA expression profiles between cAMP-stimulation and cAMP-stimulation under PGC-1a-knockdown. Overall design: ESCs were transfected with control or PGC-1a siRNA and then treated with or without cAMP for 4 days. The mRNA-seq libraries were generated. After the quality of the library was validated using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA), the samples were subjected to sequencing with a next-generation sequencer (Genome Analyzer GAIIx, Illumina, San Diego, CA). Gene expression values were calculated as “reads per kilobase of exon unit per million mapped reads” (RPKM).