Transcriptome differences between immortalized brown preadipocytes treated with different paracrine FGFs

We analyzed coding transcript abundance in primary, immortalized brown preadipocytes from murine, interscapular brown adipose tissue. We prepared primary preadipocytes from the stromal-vascular fraction of murine interscapular brown fat of 129SvEv mice and immortalized these by the SV40 large T antigen method. Cells were treated with 5.56nM Fgf1, Fgf8b or FGF9 for 48h in the presence of 1µg/ml heparin. One group of Ffg8b treated cells was also treated with 2µM Bay-876. We sequenced total RNA and determined transcript abundance.