CRC micrometastasis generated by AKP MTOs resolved by single cell RNAseq. CRC micrometastasis generated by AKP MTOs resolved by single cell RNAseq

Longitudinal single cell RNA-expression analysis of tumor cells along metastatic relapse. AKP mouse tumor organoids (MTOs) were implanted in the caecum and left to metastasize into the liver. Livers were collected at different timepoints post-injection and GFP+ CD45- cells were sorted in 96-well smart-seq plates. Tumor cells were collected from liver with colorectal cancer (CRC) micrometastatic stage disease.