Transcriptional impacts of Myc deficiency in murine bone marrow NK cells

Despite the function of Myc oncogene has been extensively studied, little is known concerning its role in Natural killer (NK) cells. By performing RNA-sequencing analyses comparing Myc-deficient to Myc-sufficient NK cells, we aim at determining the biological function of Myc in these innate lymphocytes. Overall design: To this aim we generated Ncr1cre Mycfl/fl mice, in which Myc expression is deleted in NK cells at the immature stage. We sorted the immature CD27SP NK (sorted as NK1.1+CD27+CD11b-CD3/19-) cells from the bone marrow of the Ncr1cre Mycwt/wt (WT) or Ncr1cre Mycfl/fl (KO) mice and subjected them to RNA-sequencing analysis. Ncr1cre Mycfl/fl and Ncr1cre Mycwt/wt mice were generated by crossing Ncr1cre and Mycfl/fl mice which were previously described (Narni-Mancinelli et al., 2011, 10.1073/pnas.1112064108; Trumpp et al., 2001, 10.1038/414768a). All mice were backcrossed multiple times on a C57BL/6 background.