Identification of NAC094-targeted genes using DNA affinity purification sequencing (DAP-seq)

To identify binding sites and nodule SAGs that are directly targeted by NAC094, we used DAP-seq, which allows the capture of the NAC094 regulatory targets at the whole-genome scale. A total of 2,819 binding peaks corresponding to 2,721 genes were identified from two repeats of the DAP-seq experiment. Overall design: Two repeats of the DAP-seq experiments (NAC094-1 and NAC094-2) were performed. Enriched peaks were identified by comparing the results of NAC094-1 and NAC094-2 with the control (NAC094-input).