Novel Megaptera novaeangliae (Humpback whale) haplotype reference genome

The sequencing of a kidney sample (KW2013002) from a stranded Megaptera novaeangliae (Humpback whale) calf is the first chromosome level reference genome for this species. The calf, a 457 cm and 2,500 lbs male, was found stranded in Hawai’i Kai, HI, in 2013 and was marked as abandoned/orphaned. In 2023, 1g of  kidney was sequenced  with PacBio long-read DNA sequencing, chromatin conformation capture (Hi-C), RNA sequencing, and mitochondrial sequencing to comprehensively characterize the genome and transcriptome of M. novaeangliae. The reference genome was compared to the preexisting M. novaeangliae scaffold to determine assembly improvements. Data validation includes a synteny analysis, mitochondrial annotation, and a comparison of BUSCO scores (scaffold v. reference genome and Balaenoptera musculus (Blue whale) v. M. novaeangliae). BUSCO analysis was performed on an M. novaeangliae scaffold-level assembly to determine genomic completeness of the reference genome, with a scaff..., Sample Information
A kidney sample (KW2013002) was collected from a M. novaeangliae calf on January 15, 2013, in Hawai’i Kai, HI, and deposited at the National Institutes of Standards and Technology (NIST). The sample was not collected by the authors so information regarding collection is limited to that presented herein. The calf, a 457 cm and 2,500 lbs male at the time of necropsy, was first observed on January 14, 2013, in shallow water and died between January 14 and January 15, 2013, via stranding. The calf was marked as abandoned/orphaned. In 2023, 1g of KW2013002 was sampled for sequencing by Cantata Bio.
PacBio long reads DNA sequencing
Quantification of DNA samples was performed using the Qubit 2.0 Fluorometer. For the construction of the PacBio SMRTbell library, targeting an insert size of approximately 20kb, the SMRTbell Express Template Prep Kit 2.0 was employed following the manufacturer's recommended protocol and default settings. The library was subsequently prepared for ..., , # Novel Megaptera novaeangliae (Humpback whale) haplotype reference genome

[https://doi.org/10.5061/dryad.dv41ns271](https://doi.org/10.5061/dryad.dv41ns271)

## Description of the data and file structure

The sequencing of a kidney sample (KW2013002) from a stranded Megaptera novaeangliae (Humpback whale) calf is the first chromosome level reference genome for this species. The calf, a 457 cm and 2,500 lbs male, was found stranded in Hawai’i Kai, HI, in 2013 and was marked as abandoned/orphaned. In 2023, 1g of  kidney was sequenced  with PacBio long-read DNA sequencing, chromatin conformation capture (Hi-C), RNA sequencing, and mitochondrial sequencing to comprehensively characterize the genome and transcriptome of *M. novaeangliae*. The reference genome was compared to the preexisting *M. novaeangliae *scaffold to determine assembly improvements. Data validation includes a synteny analysis, mitochondrial annotation, and a comparison of BUSCO scores (scaffold v. reference genome and *...