Competitive inhibition of biotinylated CII259–273 binding to the Aq protein by glycopeptides 1–21 and responses by Aq-restricted T-cell hybridomas.

aData are expressed as the percentage of biotinylated CII259–273 bound in the absence of competitor glycopeptide (mean values of triplicates ± one standard deviation). Glycopeptides assigned with – were classified as inactive (File S1).bThe magnitude of the T-cell responses were determined from the concentration of antigen required to induce secretion of IL-2 corresponding to 10% of the measured max response for glycopeptide 1: − = no response, + = 150 µM, ++ = 30 µM, +++ = 6.0 µM, ++++ = 1.2 µM, +++++ = 0.24 µM, ++++++ = 0.0064 µM, n.d. = not determined. T-cell hybridomas were selected from groups with different specificity for the hydroxyl groups on the GalHyl264 moiety [8].