Proteomic analysis of barley mapping population subjected to drought identifies proteins with genotype×environment interaction and pQTLs

Drought is one of the major abiotic stresses negatively influencing crop yield and is a serious issue in modern agriculture. To achieve further substantial crop improvements in terms of drought resistance it is necessary to incorporate scientific results into breeding strategies. However, most of the data on plant drought responses arises mostly from small-scale studies and, therefore, its use in breeding programs is very limited. Here, we present the results of the large-scale proteomic analysis performed on barley recombinant inbred lines (RILs) and their parental genotypes subjected to drought, applied shortly before tillering. The conducted proteomic analyses enabled us to monitor drought-induced proteome changes in leaf and root tissue, and to identify proteins that responded to drought in a genotype-specific manner, for instance Rubisco activase, luminal binding protein, phosphoglycerate mutase, glutathione S-transferase, heat shock proteins as well as enzymes involved in phenylpr...