High throughput sequencing of targeted mutagenesis

NIAID Data Ecosystem2026-05-26 收录

靶向诱变测序

定向进化

数据链接：

https://www.ncbi.nlm.nih.gov/sra/SRP156317 数据链接链接失效反馈

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资源简介：

High throughput sequencing of loci in E. coli that are targeted with genetic diversifiers

创建时间：

2018-08-03

催化元件库的序列数据

定向进化

合成生物学

催化元件库数据集包含了酶催化元件定向挖掘和酶催化元件多目标协同进化数据。通过在土壤中对腈水解酶、腈水合酶等多种酶进行挖掘，将有活性的酶进行基因测序并克隆到表达载体上；通过在数据库对包含腈水解酶、烯烃还原酶、醛酮还原酶、羰基还原酶等在内的多种酶进行筛选，委托生物公司合成酶基因序列，克隆到表达载体上；通过设计的引物对醛酮还原酶、羰基还原酶等在内的多种酶进行定点突变，构建出催化性能增强的酶序列。数据采集

国家基础学科公共科学数据中心130

Replicative RNA enables directed evolution and Darwinian adaptation in mammalian cells

定向进化

哺乳动物细胞

This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

NIAID Data Ecosystem50

Rational Design and Directed Evolution of E. coli Apartate Aminotransferase to Tyrosine Aminotransferase: Mutant P2.

蛋白质工程

定向进化

Rational Design and Directed Evolution of E. coli Apartate Aminotransferase to Tyrosine Aminotransferase: Mutant P2.

NIAID Data Ecosystem10

Directed evolutionary changes in Kemp Eliminase KE07 - Crystal 4 Wild Type

定向进化

蛋白质晶体

Directed evolutionary changes in Kemp Eliminase KE07 - Crystal 4 Wild Type Descriptor: De novo designed kemp eliminase KE07 Authors: Jackson, C.J, Hong, N.-S, Carr, P.D. Deposit date: 2015-08-06 Relea

Protein Data Bank Japan2023-09-27 更新70

Thymidine kinase mutants obtained by random sequence selection.

定向进化

抗病毒药物代谢酶工程

Knowledge of the catalytic properties and structural information regarding the amino acid residues that comprise the active site of an enzyme allows one, in principle, to use site-specific mutagenesis

PubMed Central1993-05-01 更新40

High throughput sequencing of targeted mutagenesis

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