Single cell RNA-seq analysis of novel splenic DC

To understand the cellular diversity within CD11b+ splenocytes between control and GM-CSF-treated mice, we performed the CITE-seq in control and GM-CSF treated mice. FACS sorted CD11b+ splenocytes were loaded into the Chromium system (10x Genomics, Pleasanton, CA, USA) targeting 7,000 cells per sample. The cDNA libraries for mRNA were generated using Chromium Single Cell 3' v3 Reagent Kits according to the manufacturer’s instruction. Following the CITE-seq protocol, ADT PCR additive primers were added to cDNA PCR and the ADT libraries were generated separately from the mRNAs.