Supplementary file 1_The value of unsupervised machine learning algorithms based on CT and MRI for predicting sarcopenia.doc

ObjectivesThis study aims to investigate the efficacy of unsupervised machine learning algorithms, specifically the Gaussian Mixture Model (GMM), K-means clustering, and Otsu automatic threshold partitioning, in predicting sarcopenia based on computed tomography (CT) and magnetic resonance imaging (MRI) data.
MethodsA retrospective analysis was conducted on a dataset comprising 191 patients diagnosed with sarcopenia and 327 control patients. Participants were randomly assigned to training and validation cohorts in a 6:4 ratio. The paravertebral muscles at the lumbar 3/4 intervertebral disc level were manually delineated as the region of interest (ROI) on non-enhanced CT and axial T2-weighted MRI images. Muscle and adipose tissues were automatically segmented from the ROI using GMM, K-means, and Otsu algorithms at the cohort level. Quantitative metrics such as mean, volume, and volume percentage were computed, and these parameters were compared between the sarcopenia and non-sarcopenia groups. Logistic regression analysis was employed to develop predictive models for sarcopenia, with model performance evaluated using the area under the curve (AUC). The stability of the models was assessed through five-fold cross-validation.
ResultsThe study demonstrates that three unsupervised clustering algorithms utilizing CT data surpassed those employing MRI data. Notably, the CT-based Otsu model exhibited the highest predictive performance in both training and validation datasets, with AUC values of 0.986 and 0.958, respectively. This was followed by the CT-based GMM, which achieved AUC values of 0.990 and 0.903, and the K-means model, with AUC values of 0.727 and 0.772. Furthermore, the CT-based GMM model demonstrated superior stability upon five-fold cross-validation, yielding an average AUC of 0.990.
ConclusionThe findings indicate that CT-based unsupervised machine learning models outperform their MRI-based counterparts, with the CT-based Otsu and GMM models showing exceptional efficacy in sarcopenia prediction, as evidenced by AUC values exceeding 0.95.