Expression data from intestine of HDAC1 and HDAC2 conditionally mutated mice

Acetylation and deacetylation of histones and other proteins depend on the opposing activities of histone acetyltransferases and histone deacetylases (HDACs), leading to either positive or negative gene expression changes. The use of HDAC inhibitors (HDACi) has uncovered a role for HDACs in the control of proliferation, apoptosis and inflammation. However, little is known of the roles of specific HDACs in intestinal epithelial cells (IEC). We investigated the consequences of ablating both Hdac1 and Hdac2 in murine IECs gene expression. HDAC1 and HDAC2 conditionally mutated mice were provided by Dr EN Olson (University of Texas Southwestern Medical Center, Dallas, TX) (Montgomery et al., 2007). Floxed HDAC1 and HDAC2 mice were crossed with villin-Cre transgenic mice to insure specific intestinal epithelial cell gene deletion (Madison et al., 2002). Total RNAs from the colon of three control and three HDAC1/2 IEC-specific knockout mice were isolated with the Rneasy kit (Qiagen, Mississauga, ON, Canada).组蛋白及其他蛋白质的乙酰化与去乙酰化过程，依赖于组蛋白乙酰转移酶与组蛋白去乙酰化酶（HDACs）的拮抗活性，进而引发基因表达的正向或负向调控变化。借助组蛋白去乙酰化酶抑制剂（HDACi）的相关研究已证实，HDACs在细胞增殖、凋亡及炎症调控中发挥重要作用。然而，目前对于特定HDAC亚型在肠上皮细胞（IEC）中的具体功能仍知之甚少。本研究针对小鼠肠上皮细胞中同时敲除Hdac1与Hdac2基因对基因表达的影响展开了系统探究。HDAC1与HDAC2条件性敲除小鼠由美国德克萨斯大学西南医学中心达拉斯分校的EN Olson博士提供（Montgomery等，2007）。将携带loxP位点的HDAC1和HDAC2小鼠与villin-Cre转基因小鼠交配，以确保实现肠上皮细胞的特异性基因敲除（Madison等，2002）。采用Rneasy试剂盒（Qiagen，加拿大安大略省密西沙加市），从3只对照组小鼠与3只HDAC1/2肠上皮细胞特异性敲除小鼠的结肠组织中提取总RNA。