Additional file 1 of GIF1 controls ear inflorescence architecture and floral development by regulating key genes in hormone biosynthesis and meristem determinacy in maize
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Additional file 1: Supplemental Figure 1. Identification of total proteins and immunoprecipitated proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Supplemental Figure 2. Summary of chromatin immunoprecipitation sequencing (ChIP-seq). Supplemental Figure 3. Targets of GIF1 detected by chromatin immunoprecipitation sequencing (ChIP-seq). Supplemental Figure 4. Schematic diagram of the gif1 over-expression construct. The construct components include the T-DNA right border, RB; and left border, LB; CaMV35S promoter, CaMV35S; terminator of nopaline synthase gene, tnos; enhanced green fluorescent protein gene, eGFP; the phosphinothricin acetyltransferase cassette, bar. Supplemental Table 1. Conservation of identified proteins in Arabidopsis, maize leaf and maize ear. Supplemental. Table 2. Putative GIF1-bound targets identified by ChIP-seq and RNA-seq. Supplemental Table 3. Primer sequences used in this study.



