Supplementary Tables: A qPCR-based method for rapid quantification of six intestinal homeostasis relevant bacterial genera in feces

Supplementary
Table 1. Bacterial species and strains used for six genus-specific
primer pairs designa
a16S
rRNA sequences of above species or strains covering Bacteroides, Lactobacillus, Pediococcus, Enterococcus,
Streptococcus, Bifidobacterium, Prevotella,
Clostridium,
Eubacterium, Ruminococcus, Leuconostoc, E. coli and Klebsiella pneumoniae were retrieved from the NCBI GenBank database, and
aligned to determine the regions designed for primers. The bacteria in the
same box were closely
related.

Supplementary
Table 2. Level of similarity for 16S rRNA gene of 13 bacterial genera
mentioned in Table S1.

16S rRNA gene sequence of 13 different bacterial
genera used in Table S1 were aligned with each other, respectively. The
similarity levels were detected by DNAMAN8.0.

Supplementary
Table 3. Comparison between
available primers and novel primers designed for six bacterial genera in this
study.

补充表 1. 用于设计六组属特异性引物的细菌物种和菌株。

a16S rRNA（细菌16S核糖体RNA）序列，涵盖上述物种或菌株的 Bacteroides、Lactobacillus、Pediococcus、Enterococcus、Streptococcus、Bifidobacterium、Prevotella、Clostridium、Eubacterium、Ruminococcus、Leuconostoc、E. coli 及 Klebsiella pneumoniae，均从 NCBI GenBank 数据库中检索并比对，以确定引物设计所针对的区域。同一方框内的细菌彼此密切相关。

补充表 2. 表 S1 中提及的 13 种细菌属 16S rRNA 基因的相似度水平。

将表 S1 中使用的 13 种不同细菌属的 16S rRNA 基因序列相互比对，并通过 DNAMAN8.0 检测相似度水平。

补充表 3. 本研究中为六种细菌属设计的引物与现有引物的比较。