Phospho-proteomic profile of control and Palbociclib-treated HeLa cells

Liquid Chromatography with tandem mass spectrometry (LC-MS/MS) identification of phosphorylation of control and Palbociclib-treated HeLa cells. For identification and quantification of protein, the single-shot subject samples (DIA MS data) were used to generate a direct-DIA library and computationally merged into a hybrid library in the Spectronaut software (Biognosys). For subsequent data analysis, Gene Ontology (GO) and InterPro (IPR) analysis were conducted using the InterProScan-5 program against the non-redundant protein database, and the databases COG (Clusters of Orthologous Groups) and KEGG (Kyoto Encyclopedia of Genes and Genomes) were used to analyze the protein family and pathway.