Integrative genomic strategies applied to a lymphoblast cell line model reveal specific transcriptomic signatures associated with clozapine response [expression]

In vitro genomic analyses of clozapine response. Genome wide gene expression profiling was performed across several experimental conditions of clozapine exposure. LCLs were exposed for 24 hours to 1x, 10x, 50x and 100x clinical concentration (2μM, 20μM,100μM,200 μM clozapine) and vehicle (DMSO); each concentration was measured in 4 cell lines. Lymphoblast cells were lysed and RNA extracted using column-based extraction methods from Qiagen according to manufacturer’s instructions. Gene expression profiling was carried out using Illumina HumanHT- 12 v4 Expression BeadChip technology.