Reverse MR analysis data.

Idiopathic sudden sensorineural hearing loss (ISSNHL) is defined by the rapid onset of hearing impairment without an identifiable etiology. The decreased serum concentrations of 25-hydroxyvitamin D(25(OH)D) was shown to be associated with decreased hearing ability. However, current cross-sectional studies have only demonstrated an association, failing to establish a causal link. Therefore, a comprehensive investigation is necessary to clarify the causal relationship between them. Analysis was done by using largescale genome-wide association analysis(GWAS) summary datasets to give information about the incidence of ISSNHL and genetically predicted serum 25(OH)D levels by a two-sample Mendelian randomization (MR) analysis. Instrumental variables (IVs) were identified as genome-wide significant single-nucleotide polymorphisms (SNPs) with a significance threshold of P −8 and an independence criterion of r2 P −8). Our results indicated no causal association between serum 25(OH) D levels and the risk of ISSNHL within the European population (IVW; OR = 1.09, 95% CI = 0.81 to 1.48, P = 0.573). Furthermore, the statistical models did not reveal any evidence of heterogeneity or pleiotropy.