M. bovis proteins identified by mass spectrometry and reactions with sera from naturally infected cattle in immunoblotting experiments.

Protein spots from 2-DE were sequenced using MALDI-TOF/TOF MS and identified by searching mycoplasma databases using the MASCOT search engine 2.2. A GPS explorer protein confidence index ≥95% were used for further manual validation.aC.I. %: the confidence interval for the protein score.bCOG database functional classes: (C) energy production and conversion, (D) cell cycle control, cell division, chromosome partitioning, (E) amino acid transport and metabolism, (F) nucleotide transport and metabolism, (G) carbohydrate transport and metabolism, (H) coenzyme transport and metabolism, (J) translation, ribosomal structure and biogenesis, (O) post-translational modification, protein turnover, chaperones.