Transcriptional profiling of ageing muscle satellite cells treated with recombinant human MG53

Purpose: Skeletal muscle satellite cells (SCs) are essential for muscle regeneration. MG53 (TRIM72) is a key regulator of membrane repair, but its role in SC fate decisions remains elusive. This study aims to investigate the transcriptional regulatory role of MG53 in satellite cell quiescence maintenance, activation, and proliferation. Methods: Primary satellite cells were isolated from hindlimb muscles of C57BL/6 mice. Cells were cultured and treated with either recombinant human MG53 protein (rhMG53) or BSA (Vehicle Control) for 3 Days. Total RNA was extracted and subjected to high-throughput RNA sequencing (RNA-seq) to identify differentially expressed genes (DEGs). Results: Our analysis reveals that MG53 treatment significantly alters the transcriptomic landscape of satellite cells. Specifically, MG53 treatment upregulated genes associated with activation pathways, while downregulating genes related to inflammation or lineage differentiation. Conclusions: This dataset provides novel insights into the molecular mechanisms by which MG53 promotes satellite cell maintenance and regenerative capacity, highlighting its potential therapeutic value for muscle wasting diseases. Overall design: Primary satellite cells were isolated from C57BL/6 mice. Cells were cultured in growth medium and treated with either rhMG53 protein or BSA (as vehicle control) for 3 days. Total RNA was harvested from 3 biological replicates per group (Total N=6). Comparative gene expression analysis was performed to identify differentially expressed genes (DEGs) between MG53-treated and Control groups.