Supplemental Tables and Excel files for lifelong exercise transcritpome responses to exercise in slow and fast muscle fibers.

We investigated fast and slow muscle fiber transcriptome exercise dynamics among three groups of men: Lifelong exercisers (LLE, n=8, 74±1 y), old healthy non-exercisers (OH, n=9, 75±1 y), and young exercisers (YE, n=8, 25±1 y).  On average, LLE had exercised ~4d•wk-1 for ~8h•wk-1 over 53±2 years.  Muscle biopsies were obtained pre- and 4h post-resistance exercise (3x10 knee extensions at 70% 1-RM).  This study provides the first insights into fast and slow muscle fiber transcriptome dynamics with lifelong endurance exercise.  The fast fibers were more responsive to exercise with divergent transcriptome signatures among young exercisers (growth and metabolic), lifelong exercisers (metabolic) and old healthy non-exercisers (stress).  Only lifelong exercisers had a biological response in slow fibers (metabolic).  These data provide novel insights into fast and slow muscle fiber health at the molecular level with age and exercise.  The study was approved by the Institutional Review Board at Ball State University.  All study procedures, risks, and benefits were explained to the subjects before giving written consent to participate.