Dataset: 1H NMR metabolomic study of auxotrophic starvation in yeast using Multivariate Curve Resolution-Alternating Least Squares for Pathway Analysis

This dataset contains the set of 1H NMR data used in https://doi.org/10.1038/srep30982.

Yeast was grown in five different liquid media and their metabolism was characterized at 6 different time-points during 24 h.

The media used were YSC (Yeast nitrogen base Synthetic Complete) and four Drop-Out (DM) medium that do not contain one of the following nutrients (L-histidine, L-leucine, L-methionine and uracil). Since the used yeast strain does not encode in its genome some genes relative to the biosynthesis of these four nutrients, some gene de-regulations process will occur, detectable at the metabolome level.

In this study, we have characterized the metabolome using 1H NMR spectroscopy, detecting more than 40 metabolites, and the evolution of this metabolome along the measured time-points was described by application of PCA, ASCA and MCR-ALS chemometric methods.