Expression profile of microRNAs in different stages of wound healing in mice

Wound healing response is characterized by progression from clot formation
to an inflammatory phase, a repair phase, and finally
remodeling. MicroRNAs (miRNAs) are 20-24 nucleotide noncoding
RNAs that can affect protein expression at the posttranscriptional level
by modulating the stability and translation of corresponding messenger
RNAs (mRNAs). The roles that miRNAs play in various physiological and
pathological processes have received increasingly widespread
attention. However, the differentially expressed miRNAs involved
in different stages of wound healing and their roles remain to be further
explored. In this study, we established wound model on the shaved
dorsum using a punch biopsy tool in C57BL/6J mice. Biopsies were
harvested on postoperative days 0, 1, 3, and 7. Then, we
identified the differentially expressed miRNAs
in different stages of wound healing through Illumina sequencing
and miRDeep2 analysis. This study help to find out the expression profile
of microRNAs during normal wound healing in mice.