Hap complex regulates the development of Penicillium oxalicum fungi and the expression of cellulolytic genes

The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT) and Hap3/5 deletion strain (Dhap3/5) under conditions of 2% glucose and 1% bran + 1% microcrystalline cellulose as carbon source. The results of correlation analysis between each sample showed that the gene expression level of Dhap3/5 was significantly different from that of the WT strain. The deletion of hap3/5 significantly affect the expression levels of sporulation process genes and the expression of cellulase-encoding genes. Overall design: Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain and hap3/5 deletion mutant strains in 24h in modifeid Mendal's medium with 2% glucose or 1% bran + 1% microcrystalline cellulose as carbon sources. Three replicates were included for each samples.