Effect of hormonal therapy on the otoconial changes caused by estrogen deficiency

To examine the effect of hormonal therapy on otoconial changes caused by estrogen deficiency, we dissected the utricle and extracted its total RNA. We performed gene expression analysis using data obtained from RNA-seq of four groups. Overall design: Comparative gene expression analysis of RNA-seq data for sham operation with vehicle, ovariectomy with vehicle, ovariectomy with estradiol, and ovariectomy with raloxifene.