NG-Capture C analysis of 35 gene loci regulated by Fra-1 in MDA-MB-231 cells

In the article "Fra-1 regulates its target genes via binding to remote enhancers without exerting major control on chromatin architecture in triple negative breast cancers" by Bejjani et al., we used NG Capture-C approach to identify regulatory elements interacting with the promoters of 35 Fra-1 regulated genes in the triple negative breast cancer cell line MDA-MB-231 Overall design: Identification of chromatin interactions by NG Capture-C between distal regulatory elements and the promoters of 35 Fra-1 regulated genes in MDA-MB-231 cells in presence and absence of Fra-1 (siRNA approach) The 35 assayed genes include: ABTB2 ASB1 CD44 CDCP1 EDN1 EMP1 FNDC3B FOSL1 GALNT2 HEG1 HES1 HK2 HMGA1 ID1 IL8 KRT80 LIPH LRP10 MAP2K1 MXD1 MYO10 PDE2A PITPNC1 RPSAP52 SMAD6 SOX9 SRGAP1 SSFA2 TCF7L2 TES TGFB2 TMC6 TNS3 TSPAN2 ZNF114