The whole transcriptome RNA sequencing of tumor tissue samples from 61 pancreatic ductal adenocarcinoma patients.. The whole transcriptome RNA sequencing of tumor tissue samples from 61 pancreatic ductal adenocarcinoma patients.

Tissue samples were collected from 61 stage I/II PDAC cases then were placed into 10% neutral buffered formalin solution, fixed at roomtemperature (8–72 h) and processed routinely into a paraffin embedded tissue block (FFPE). The RNAstorm™ Kit (biotium, USA) was used to extracted and isolated total RNA fraction from FFPE Tissue Samples. RNA libraries were prepared for sequencing using prepared SMARTer® Stranded Total RNA-Seq Kit - Pico Input Mammalian Kit. RNA-seq was performed by the Illumina sequencing platform on a 150-bp paired-end run. Overall design: The RNA expression profiles of formalin-fixed, paraffin-embedded PDAC tissue samples by whole transcriptome sequencing.