Chan-Soo Lee, Chang-Ki Choi, Eun-Young Shin, Martin Alexander Schwartz, Eung-Gook Kim (2011) CIL:26545, Mus musculus, fibroblast. CIL. Dataset

To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, NIH3T3 cells were treated with scrambled siRNA (Scr) for 2 days, treated with DMSO for 30 min., and stained for βPIX (green) and actin (red). These findings help elucidate a functional link between MII and Rac1/Cdc42 GTPases, which may regulate protrusion/adhesion dynamics in migrating cells. This image is the original data file from Fig. 6B, “Requirement for βPIX in MII-regulated cell protrusion and adhesion.” J. Cell Biol. 2010. Vol. 190(4):663–674.

为探究非肌肉肌球蛋白II（MII）调节迁移细胞突起和粘附动力学的作用机制，研究人员将NIH3T3细胞用打乱序列的siRNA（Scr）处理2天，并用二甲基亚砜（DMSO）处理30分钟，随后对βPIX（绿色）和肌动蛋白（红色）进行染色。这些发现有助于阐明MII与Rac1/Cdc42 GTP酶之间的功能联系，这些酶可能调节迁移细胞中的突起/粘附动力学。本图是图6B，“βPIX在MII调节的细胞突起和粘附中的必要性”的原数据文件。J. Cell Biol. 2010. 第190卷（第4期）：663–674。