Real-time quantitative PCR analysis of Clade A PP2C genes in Brassica juncea var. tumida of different tissues and under multiple stresses

Clade A PP2C genes in different organs and responding to stresses were screened using qPCR. The BjuACTINs were regarded as house keeping gene.To analyze the gene expression patterns of clade A BjuPP2Cs genes under biotic stress, 14-day-old seedlings were treated with P. brassicae liquid (OD600=0.07) for 0, 0.25, 0.5, 1, 3, 5, 7, and 9 days; to analyze the gene expression patterns of clade A BjuPP2Cs genes under abiotic stresses, 5-day-old seedlings were treated with 50 μM ABA, 100 mM NaCl and 4℃ treatment for 3 h. To analysis the gene expression level in different growth stages of stem swelling, the samples D1 (the stems of 1-month-old seedlings, six leaf stage), D2 (the stems of 2-month-old seedlings, primary stage of stem swelling), D3 (the stems of 3-month-old seedlings, early stage of stem swelling), D4 (the stems of 4.5-month-old seedlings, fast-growing stage of stem swelling) and D5 (the stems of 5-month-old seedlings, last stage of stem swelling) of B. juncea var. tumida which were grown in the field were collected. The experiment was designed to gain insight into the temporal and spatial transcription patterns of 20 Clade A PP2C genes in tuber mustard.The experiment was designed to examine the transcription patterns of 20 Clade A PP2C genes in different organs and different stages of stem swollen, as well as response to 50 μM ABA, 100 mM NaCl and 4℃ treatment for 3 h, and response to the pathogen Plasmodiophora brassicae in different time points.