Faraguna_Pena_Kantarci_STAR Protocols

RNAscope images shows the expression of Nav1.6 (C3), Nav1.7(C1) and Nav1.8(C2) in DRG neurons isolated using the protocol described here, cultured for 9 days in vitro (DIV), and either treated with DMSO (0.01%) or 1 uM 16,16-dimethyl PGE2 (dmPGE2)—a PGE2 derivative that is resistant to degradation on DIV8 for 16-24 h. dmPGE2 increases the expression of indicated voltage-gated sodium channels as discussed in our referenced publication ( PMID: 39142281). These images are added in relation to our protocol that describes purification of dorsal root ganglion neurons in highly pure cultures. Isolated DRG neurons can be stained using in situ hybridization, RNAscope, as shown in these example images.