Supplemental Material for Randhawa et al., 2020

<b>Table S1</b> Comparison of SNP-based current poll testing (CPT) and the optimized poll testing (OPT) assays on 20,636 samples for the frequency of “No results” in various cattle breeds. <b><br></b><b>Table S2</b> List of 14 microsatellites mapped within the Poll locus on chromosome 1. <b><br></b><b>Table S3</b> Call rate of 10 SNPs in different cattle breeds and cross-bred populations. <b><br></b><b>Table S4</b> Results of targeted DNA sequences of 55 Brahman samples showing 60bp genome region around SNP rs800947704[C/T=Y] (marked as 0) and upstream variant SNP rs381418143 [A/G=R] (marked as -3). Two types of errors were observed in the genotyping assays, 1: genotype failed (--) when -3=G (yellow highlights), 2: wrong genotypes when -3=R and 0=Y (purple highlights). <b><br></b><b>Figure S1</b> Distribution (%) of MSAT and SNP based poll gene testing results for Horned (HH), Hybrid (HP), Polled (PP) and failing (“Not Determined” or “No Results” ) samples across 10 Australian beef breeds (n=16,941). <b><br></b><b>Figure S2</b> Breed-wise distribution (%) of Optimized Poll Testing (OPT) assay results (HH = Horned, HP = Hybrids, PP = Polled, No Results = Failed assay) in first column and Allele Frequencies (AF) of Horn (H), Poll-Celtic (P_C) and Poll-Friesian (P_F) mutations in column 2 across ten Australian beef breeds (n = 16,903). Note that presence of H alleles in Angus and P_C/P_F alleles in Wagyu is indicative of cross-bred individuals. Overall, the results show that polled animals of Australian beef breeds predominantly carry P_C as compared to P_F, except for Shorthorn. <b><br></b><b>Data 1</b> Validation population phenotypes and Poll markers genotypes data file: Data_1_project_validation_populations_phenotypes_and_poll_locus_SNPs.csv <b><br></b><b>Data 2 </b>Commercial population Poll markers genotypes and BREEDPLAN phenotypes data file: Data_2_project_poll_locus_10_SNPs_genotypes_and_BREEDPLAN_phenotypes.csv