Feasibility of label-free phosphoproteomics and application to base-line signaling of colorectal cancer cell lines.

Data from ProteomeXchange, PXD ID: PXD001550. File: QE2_130117_OPL1013_TiOx_CRC_DLD1_3_130125023714.mzml. Published as part of J Proteomics. 2015 Apr 1. pii: S1874-3919(15)00103-7 . From the Abstract: {{i}} Robust phosphopeptide enrichment methods with minimal fractionation are required to profile signaling network analysis in cancer cell lines and tissues. We assessed performance of single-shot LC-MS/MS label-free phosphoproteomics using TiOx-based phosphopeptide enrichment and report phosphopeptide identification reproducibility (75.8%), depth of identification (6014-6150 phosphopeptides) and reproducibility of label-free quantification (CV 17.8%). Subsequently, we have profiled the baseline global phosphorylation of 8 colorectal cancer (CRC) cell lines representing different CRC prognostic subtypes ... {{/i}}