Effect of organ bath washout after 60 min of cyclic stretching on bronchial basal tone and responsiveness.

To eliminate the possible stretch-induced secretion of contractile mediators into the organ bath, a washout was performed (complete renewal of the Krebs-Henseleit solution) after 60 min of cyclic stretching. Data are quoted as the mean ± SEM, the standardized effect size (d) and latter's 95% confidence interval (CI) for the difference between means. ACh, acetylcholine; ΔEmax (efficacy), difference between the maximum contraction obtained with 3 × 10–3 M ACh and the basal tone recorded just before the second dose-response curve was established (Fig 2);-log EC50 (potency), negative log of the ACh concentration producing 50% of the maximum effect (second dose-response curve) (Fig 2). Compared with paired no washout controls, washout did not change the post-stretching basal tone or ACh's efficacy and potency.Effect of organ bath washout after 60 min of cyclic stretching on bronchial basal tone and responsiveness.