Microarray expression profiles of circular RNA in malignant pleural effusion

To explore the expression pattern of circular RNAs (circRNAs) and their biological functions in malignant pleural effusion, we surveyed the circRNA expression profiles of 3 lung adenocarcinoma-associated malignant pleural effusion (LA-MPE) and 3 tuberculous pleural effusion (TPE) from clinical patients using Clariom D human microarray. The pleural effusions were divided into two experimental groups: test group (LA-MPE) and control group (TPE). After being centrifuged, the cell pellets were resuspended in TRIzol reagent to extract the total RNA. CircRNA expression profiles were then explored by Clariom D human microarray from Affymetrix.