RNA-seq analysis of MRA_3376 mutant in mycobacterium tuberculosis H37Ra

we compared the global transcription profile between the MRA_3376 mutant and wild type strain using RNA sequence technology. MRA_3376 mutant and wild type strains were grown in 7H9 to mid logarithmic phase and were harvested by centrifugation. Total RNA was extracted using an RNeasy mini kit. Library constructions were prepared using TruSeq Stranded Total RNA Sample Preparation kit and RNA sequencing was conducted using an Illumina HiSeq2500. The inser size conformation of purified libraries was validated by an Agilent 2100 bioanalyzer. Then, HTSeqv2.1.1 was run with a reference annotation to generate fragments per kilobase of exon model per million mapped reads values for estimation of fold changes. Three biological replicates were used in RNA sequence.