Intratracheal administration of TIP to PVM-infected C57BL/6 mice in different dose regimens and time schedules did not have an effect on pulmonary edema formation.

A: Lung wet-to-dry weight ratio of the left lung of PVM-infected mice that received 20 µg of TIP in 30 µl saline or 30 µl saline on day seven after infection and were studied two, four or six hours later. B: Lung wet-to-dry weight ratio of the left lung of PVM-infected mice that received 80 µg of TIP in 60 µl saline or 60 µl saline (control) on day seven after infection and were studied two, four or six hours later. C: Lung wet-to-dry weight ratio of the left lung of PVM-infected mice that received 80 µg of TIP in 60 µl saline or 60 µl saline (control) on day 5 after infection and were studied 24, 48 and 72 hours later. D: Lung wet-to-dry weight ratio of the left lung of PVM-infected mice that received 20 µg TIP in 30 µl of saline or 30 µl saline on day zero, two, four and six after infection and were studied on day seven after infection. The graphs represent the mean of 4–8 mice at each time-point (± SEM).