Transcriptome analysis of the transgenic and non-transgenic fleshy roots

Effect of betanin synthesis on the metabolism of transgenic carrot. Betalain is a natural pigment with important nutritional value and broad application prospects. Previously, we produced betanin biosynthesis transgenic carrots via expressing optimized genes CYP76AD1S, cDOPA5GTS and DODA1S. Betanin can accumulate throughout the whole transgenic carrots. But the effects of betanin accumulation on the metabolism of transgenic plants and whether it produces unexpected effects are still unclear. We then performed gene expression profiling analysis using data obtained from RNA-seq of the transgenic and non-transgenic fleshy roots. In our previous study, stably transformed carrot lines containing CYP76AD1S, cDOPA5GTS and DODA1S genes can synthesize and accumulate betanin in the whole plant. In addition, metabolic changes were analyzed by transcriptome between betanin-transgenic carrots (BC) and non-transgenic carrots (WT) in this study.