Primer sequences used for quantitative RT-PCR estimation of Myotis lucifugus innate response genes.

Myotis homologues for innate response genes were identified by scanning the Myotis genome for sequences that matched those of well-characterized reference organisms. Where qRT-PCR primers for reference organismsd had been validated in the literatureb,c, we used the corresponding Myotis sequences as primers. Myotis-specific substitutions in these primers are in bolda. For other genes (HSP70, TNFα, NOS2, IL-10, GRP78) Myotis-specific primers were designed from potential Myotis exon sequences using parameters for optimal use in PCR reactions. In all cases the identity of PCR products were confirmed by sequencing. Primers were used in qRT-PCR at a final concentration of 1 µM.Primer sequences used for quantitative RT-PCR estimation of Myotis lucifugus innate response genes.