Transcriptional profiling by array of mouse cerebellar cortex during postnatal development

During the first 10 postnatal days of mouse development, Purkinje cells, neurons of the cerebellum, pass through different developmental processes of differentiation such as profound dendritic remodeling and growth, cell death and loss of their ability to regenerate their axons. To identify the genes involved in these different processes and in particular transcription factors, the gene expression patterns of murine cerebellar cortical area centered on Purkinje soma have been measured using Affymetrix microarrays at 5 different developmental stages (postnatal days P0, P3, P5, P7 and P10; 4 replicates = 4 independent measurements for each stage).在小鼠出生后发育的前10天内，作为小脑神经元的浦肯野细胞（Purkinje cell）会经历多种分化相关的发育进程，包括显著的树突重塑与生长、细胞死亡，以及丧失轴突再生能力。为识别参与这些不同进程（尤其是转录因子（transcription factor））的基因，研究人员采用Affymetrix微阵列（Affymetrix microarray），针对以浦肯野细胞胞体为中心的小鼠小脑皮层区域，在5个不同发育阶段（出生后第P0、P3、P5、P7和P10天；每个阶段设置4次生物学重复，即4次独立测量）检测了其基因表达谱。