Human oocyte maturation transcriptome

Immature oocytes were donated by patients undergoing assisted reproductive technology (ART) treatment and would otherwise be discarded. The oocytes were cultured in vitro to the appropriate developmental stages. Samples were lysed in lysis buffer. mRNA reverse transcription and amplification were performed using the SMART‑seq2 protocol with oligo(dT) priming. cDNA was amplified via the template‑switching mechanism. Amplified products were quantified and quality‑checked using a Qubit fluorometer and an Agilent 2100 Bioanalyzer. Sequencing libraries were constructed using a transposase‑based method, with fragment sizes ranging from 300 to 400 bp. Library quality and concentration were verified, and sequencing was performed on an Illumina platform with a 2×150 bp paired‑end strategy. The sample comparison information of the sequencing results is shown below：  Oocyte maturation （maturation stage： GV MI MII） Oocyte1 A2 A4 A4 Oocyte2 B1 B3 B4 Oocyte3 C2 C4 C5 Oocyte4 E2 E4 E5 Oocyte5 F1 F3 F5 Oocyte6 G1 G4 G7 Oocyte7 G2 G5 G8 Oocyte8 G3 G6 G9 Oocyte9 H3 H5 H6 Oocyte10 K2 K5 K7Oocyte fertilization (stage: MII Zygote) Oocyte 11 X2 X9 Oocyte12 X3 X10 Oocyte13 X4 X11 Oocyte14 X5 X12