Intracellular concentration of BAPTA, Ca2+ and expression of EGR1, PTGS2 and PPP1R15A in HeLa cells.
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https://figshare.com/articles/dataset/_Intracellular_concentration_of_BAPTA_Ca_2_and_expression_of_EGR1_PTGS2_and_PPP1R15A_in_HeLa_cells_/304086
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HeLa cells were incubated during 3 hrs with or without ouabain in control medium containing 1.8 mM CaCl2 (1) or in Ca2+-free medium containing 50 µM EGTA and 10 or 100 µM BAPTA-AM (2 and 3, respectively). Changes in the expression of of EGR1, PTGS2 and PPP1R15A were measured by qRT-PCR. For more details, see Methods section. Means ± S.E. obtained in experiments performed in quadruplicate are shown. ND – these values were not determined.*, ** and ***- p
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2015-12-02
