Expression (Gene) in the endothelial cells of the mouse female prefrontal cortex (PFC) following 10-day chronic social stress or 6-day subchronic variable stress exposure was compared to male [Male_SS_vs_Female_SS_or_SCVS]

Cell-specific transcriptomic analysis were performed on PFC endothelial cells of stress-susceptible (SS) and resilient (RES) mice along with females subjected to 6 days of subchronic variable stress to identify novel genes and pathways involved in stress responses. These gene lists were compared to published findings obtained in the male NAc following 10 days of chronic social stress exposure (Dudek et al., 2020) Animals were subject to either 10 days of chronic social defeat stress and phenotype deteremined using a social interaction test or 6 days of subchronic variable stress then the brains were collected 24h later and PFC punched for magnetic-activated cell sorting and transcriptomic analyses. Nac gene lists produced in Dudek et al. PNAS (2020) Genome Quebec (extracted RNA, assessed quality using the bioanalyzer, performed the Clariom S mouse assay according to the manufacturer's instructions).