Behavioral data.

AimBased on metabolomics and proteomics research, we investigate the molecular biological mechanisms underlying vascular cognitive impairment (VCI) in rats induced by hypertension combined with endothelial damage, aiming to identify proteins or metabolites associated with key metabolic pathways.MethodsSPF hypertensive rats (SHR) were used to damage the common carotid artery endothelium with microcurrent to induce vascular cognitive impairment model in rats (Model group), SHR rats (SHR group) and SPF normotensive Wistar-Kyoto rats (WKY) with the same genetic background were used as sham operation groups (no current stimulation after dissection), with 10 rats in each group. Morris water maze, PNT experiment and SPT experiment were used to detect the learning and memory ability of rats. TMT quantitative proteomics technology combined with liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to detect the difference in metabolites and proteins in brain tissue of rats with vascular cognitive impairment.ResultsFrom Day 1–5, compared with the WKY group, both the Model and SHR groups exhibited shortened incubation periods and slower average swimming speeds (P P P P ConclusionRats with hypertension combined with endothelial injury have behavioral disorders, which are similar to the causes and signs of clinical vascular cognitive impairment; the biological mechanisms manifested in the model involve amino acid metabolism disorders, energy metabolism, relaxin signaling pathway and impaired focal adhesion function. Characteristic metabolites in the model group, such as ATP, cAMP, Creatine, Cyanocobalamin, Dopamine, Serotonin, Tryptophan, Uric acid and Vitamin B1 decreased, while GABA, Glucose, Isocitrate and Malate increased.