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Expression data of siFAM210B and control A549 cells

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE87850
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Our previous study screened a novel cancer progression suppressor gene, FAM210B, which encodes an outer mitochondrial membrane protein, by the suppression of mortality by antisense rescue technique (SMART). We demonstrated that FAM210B loss was significantly associated with cancer metastasis and decreased survival in a clinical setting. We used microarrays to find diffrenet gene expression between siFAM210B and control A549 cells A549 cells were transfected with siRNA for 72h for RNA extraction and hybridization on Affymetrix microarrays, and Realtime PCR were used to verified the effect of siRNA knockdown. We used mixed siRNA, tirplicated per sample and designed the control to increase the resolution of expression profiles.
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2019-03-28
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