Gene expression profiling of small intestinal epithelia in AP-1B KO mice

To elucidate the function of AP-1B in small intestinal epithelia, which is well known as one of the components of the AP-1 clathrin adaptor complex, gene expression profiling of AP-1B KO mice was performed. Small intestine of AP-1B +/- and -/- mice were dissected and soaked in Hank's Buffered Salt Solution (HBSS) (GIBCO) containing 30 mM EDTA. After incubation on ice for 20 min, IECs were isolated by manipulation with a fine needle under stereomicroscopic monitoring (n=2).为阐明AP-1网格蛋白衔接蛋白复合物（AP-1 clathrin adaptor complex）组分之一AP-1B在小肠上皮细胞中的功能，本研究对AP-1B敲除（KO）小鼠开展了基因表达谱分析。分离AP-1B杂合型（+/-）与纯合型敲除（-/-）小鼠的小肠组织，将其浸没于含30 mM乙二胺四乙酸（EDTA）的汉克斯平衡盐溶液（Hank's Buffered Salt Solution, HBSS）（GIBCO品牌）中。于冰上孵育20分钟后，在体视显微镜监控下使用细针操作分离小肠上皮细胞（intestinal epithelial cells, IECs），每组样本量n=2。