CeA ChR2 Fos Plume worksheet

This data set shows how the spread Fos protein expression away from the fiber optic tip was quantified, and shows Fos protein levels within CeA between ChR2 and eYFP control rats among various conditions - after passive laser stimulation, after earning sucrose or cocaine paired with stimulation, or after interacting with a shock-delivering rod.Dataset supporting: Warlow, S.M., Naffziger, E.E. & Berridge, K.C. The central amygdala recruits mesocorticolimbic circuitry for pursuit of reward or pain. Nat Commun 11, 2716 (2020). https:/doi.org/10.1038/241467-020-16407-1Abstract: How do brain mechanisms create maladaptive attractions? Here intense maladaptive attractions were created in laboratory rats by pairing optogenetic channelrhodopsin (ChR2) stimulation of central nucleus of amygdala (CeA) in rats with encountering either sucrose, cocaine, or a painful shock-delivering object. Pairings made the respective rats pursue either sucrose exclusively, or cocaine exclusively, or repeatedly self-inflict shocks. CeA-induced maladaptive attractions, even to the painful shock-rod, recruited mesocorticolimbic incentive-related circuitry. Shock-associated cues also gained positive incentive value and were pursued. Yet the motivational effects of paired CeA stimulation could be reversed to negative valence in a Pavlovian fear learning situation, where CeA ChR2 pairing increased defensive reactions. Finally, CeA ChR2 valence could be switched to neutral by pairing with innocuous stimuli. These results reveal valence plasticity and multiple modes for motivation via mesocorticolimbic circuitry under the control of CeA activation.Find other related resources in the collection here: https://doi.org/10.35092/yhjc.c.4939542