Effects of estrogen and mechanical loading on cultured cells derived from mandibular condylar cartilage

Temporomandibular joint osteoarthritis is a degenerative condition influenced by mechanical loading and hormonal factors, particularly estrogen. Despite its established role in TMJ pathophysiology, the mechanisms underlying the effects of estrogen on joint cartilage remain unclear, with conflicting reports on its protective and destructive effects. This study aimed to investigate the molecular effects of estrogen and mechanical loading on cultured cells derived from the mandibular condylar cartilage, confirmed as chondrocytes via quantitative reverse transcription PCR analysis, were subjected to mechanical loading with or without estrogen treatment. RNA sequencing and bioinformatics analyses, including differential gene expression, Gene Ontology, and protein-protein interaction network analysis, identified extracellular matrix-related pathways and hub genes, such as COL1A1, COL3A1, LAMC1, and LAMB1, as key mediators. Mechanical loading reduced the expression of ECM-related genes in MCC cells, whereas estrogen combined with loading significantly upregulated COL1A1 and COL3A1 expression, as confirmed using qRT-PCR and western blotting. These results indicate this study demonstrated the effects of estrogen on mechanical loading using superficial condyle cells to elucidate the mechanisms of TMJ. Estrogen may be, at least in part, significantly involved in TMJ-OA via altering of the ECM levels such as collagen, laminin, MMPs, and ADAMTS.