RNA seq from liver samples of C57BL/6 mice to study the gene expression synchrony of Plasmodium stages with host circadian cycle.

The Plasmodium life cycle parasites differentiate and proliferate synchronously, paralleling the vertebrate host's circadian rhythm. Here we report that expression of carbohydrate metabolism and inflammatory genes in IFNγ-primed leukocytes and liver cells from infected mice are boosted in P. chabaudi-infected mice. In addition, a rhythmic gene expression of PPARG and TNFα, which are key regulators of glucose metabolism, parallel blood glucose levels across the circadian cycle in infected mice. Liver fragments from C57BL/6 mice were collected at different time points: 12 am; 6 am; 12 pm and 6 pm from mice at day 8 post-infection with P. chabaudi or uninfected control.