Kinetics of the redox reactions in STEAP1 and STEAP2

The data repository includes the kinetic data of the redox reactions in STEAP1 and STEAP2, which are presented in \"Mechanism of stepwise electron transfer in six-transmembrane epithelial antigen of the prostate (STEAP) 1 and 2\" by Kehan Chen, Lie Wang, Jiemin Shen, Ah-lim Tsai, Ming Zhou and Gang Wu. The data include: 1) the reduction of ferric STEAP1 by reduced FADH2 and ferrous STEAP2; 2) the reduction of ferric STEAP1 by cytochrome b5 reductase; 3) the reduction of ferric STEAP2 with NADPH; and 4) the oxidation of ferrous STEAP1 and STEAP2 by ferric.NTA., All the data was collected under anaerobic conditions. The time-dependent optical changes in the absorbance of the heme in STEAP1 and STEAP2 were monitored using either a diode-array UV-Vis spectrophotometer or a stopped-flow machine with a rapid-scan accessory. In each spectrum, the background absorbance is subtracted. The absorbance at 800 nm is taken as the background in the spectra acquired on the spectrophotometer and the absorbance at 703–708 nm is taken as the background in the data obtained using the stopped-flow rapid-scan method. The time courses of the Soret wavelength of the heme were either extracted from the data of the time-dependent spectral change or acquired on the stopped-flow machine operated in the single wavelength mode. 
For more experimental details, please refer to the Method section in \"Mechanism of stepwise electron transfer in six-transmembrane epithelial antigen of the prostate (STEAP) 1 and 2\" by Kehan Chen, Lie Wang, Jiemin Shen, Ah-lim Tsai, Ming Zhou and..., , # Kinetics of the redox reactions in STEAP1 and STEAP2

[https://doi.org/10.5061/dryad.00000008r](https://doi.org/10.5061/dryad.00000008r)

The kinetics data are stored in Excel files and the filenames correspond to the figures in \"Mechanism of stepwise electron transfer in six-transmembrane epithelial antigen of the prostate (STEAP) 1 and 2\" by Kehan Chen, Lie Wang, Jiemin Shen, Ah-lim Tsai, Ming Zhou and Gang Wu, https://doi.org/10.7554/eLife.88299.1. In each data file, the title of each sheet indicates the panel in the figure and the reaction.

The structures of different types of kinetic data are described as follows.

1. **Time-dependent spectral change** (Figure2, Figure2-FigureSupplement1, Figure3, Figure3-FigureSupplement1, Figure4-FigureSupplement1)
The first column is the wavelength and the spectra are listed continuously from column 2. The spectrum acquired at the first time point is always in column 2 and marked \"spectrum#1\" in the top, and the temporal sequence of the ...