Physical characteristics of DIA1 proteins and similarity to orthologues from key species.

aDIA1 homologues were restricted to the Metazoa, but were not found in the phylum Porifera or Nematoda. Partial homologues, including those found in the phylum Mollusca and Annelida (Table S2), are not shown. See Table S1 for accession numbers of full length DIA1 proteins. Only a single Drosophila species DIA1 is included here. Table S3 is an expanded version of this Table.bIsoelectric point calculated using the assumption that all residues have pKa values equivalent to that of isolated residues, and so may not accurately represent the value for the folded protein.cIsotopically-averaged molecular weight prediction in kiloDaltons.dThe BLASTP E-value measures the statistical significance threshold for protein sequence matches. The smaller the number, the better the match. Computer shorthand nomenclature is used to present E-values when values are small. For example, 5e-01 = 0.5 and 5e-04 = 0.0005. Values below 1e-250 are indicated as zero, and details of those greater than 10 are not provided. A dash is used when protein alignments have 100% identity.eProteins were compared to DIA1from H. sapiens, C. intestinalis, S. purpuratus, or D. melanogaster, or N. vectensis by protein BLAST. The DIA1 proteins used for comparison were chosen as representives from Class Mammalia, Class Aves, and Class Neopterygii within the subphylum Vertebrata, subphylum Urochordata, phylum Echinodermata, phylum Arthropoda, and phylum Cnidaria, respectively.